SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Pelizer,Lúcia Helena; Moraes,Iracema de Oliveira.

This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse...

Ação de Micoflora de efluentes agroindustriais sobre diferentes corantes e substratos lignocelulósicos - doi: 10.4025/actascibiolsci.v33i4.6187

Palavras-chave: Spirulina platensis; Solid state cultivation; Estimative of biomass; Sugarcane bagasse; Protein content.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300024

Biological Sciences

Perovano Filho, Natalino; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA; Silva, Kelly Fernanda Seára da; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA; López, Ana Maria Queijeiro; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA.

Enzimas extracelulares de fungos são importantes para sua sobrevivência em ambientes inóspitos, sendo capazes de degradar compostos recalcitrantes. Fungos filamentosos secretam hidrolases e fenoloxidases capazes de degradar complexos de estruturas aromáticas, como de alguns xenobióticos. O propósito deste trabalho foi testar diferentes corantes (Azul de Metileno, Azure B, Verde Malaquita e Cristal Violeta), em substituição ao composto Poly R478, no método de seleção de microrganismos degradadores de fenóis em meio sólido, além de comparar tal atividade de Micoflora isolada de efluentes de indústria sucroalcooleira com a de outros fungos adquiridos em coleção oficial. Os corantes testados revelaram-se ótimos substitutos do Poly R478. O isolado que...

Tipo: Por composição bioquímica;

Palavras-chave: 2.12.02.00-1 degradação biológica; Fenoloxidases; Bagaço de cana; Serragem de madeira; Azul de Metileno; Cladosporium Microbiologia Aplicada biological degradation; Phenol; Sugarcane bagasse; Sawing wood; Methylene blue; Cladosporium.

Ano: 2011

URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6187

Bioconversion of biomass: a case study of ligno-cellulosics bioconversions in solid state fermentation

BABT

Pandey,Ashok; Soccol,Carlos R..

Lignocellulosic residues obtained from crops cultivation form useful sources to be used as substrate for bioconversion processes. Sugarcane bagasse, which is a complex substrate obtained from the processing of sugar cane, is an important biomass among such sources. Due to its abundant availability, it can serve as an ideal substrate for microbial processes for the production of value added products. This paper reviews recent developments on biological processes developed on production of various products in solid state fermentation using sugarcane bagasse as the substrate and describes production of protein enriched feed, enzymes, amino acid, organic acids and compounds of pharmaceutical importance, etc. through microbial means.

Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility

Palavras-chave: Ligno-cellulosics; Sugarcane bagasse; Bioconversion; Solid state fermentation.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400001

Electron. J. Biotechnol.

Ladeira,Silvania A; Cruz,Erica; Delatorre,Andréia B; Barbosa,João B; Leal Martins,Meire Lelis.

Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as other suitable applications. Results Bacillus sp. produced cellulases when cultivated at 50°C in liquid cultures containing sugarcane bagasse and corn steep liquor. Maximum avicelase (0.83 U mL-1) and CMCase (0.29 U mL-1) activities were reached in 120 h and 168 h of culturing time, respectively. The avicelase and CMCase presented an optimum activity at pH of 7.5 and 8.0, respectively. The maximum stability of avicelase and CMCase was observed at a pH range...

Tipo: Journal article

Palavras-chave: Avicelase; Carboxymethylcellulase; Corn steep liquor; Sugarcane bagasse.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200008

Enzymes: Bioprospecting of fungi that produce enzyme complexes.

Enzymes: immobilized cellulases for hydrolysis of pretreated sugarcane bagasse

FAVARO, L. C. de L..

Elite strains of endophytic fungi, isolated from the Brazilian biodiversity, producers of efficient enzyme complexes for hydrolysis of the pre-treated sugarcane bagasse.

Tipo: Folders

Palavras-chave: Sugarcane bagasse; Enzymes; Fungi; Hydrolysis.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133089

Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2

RODRIGUES, D. de S..

Biocatalyst consisting of immobilized cellulases for hydrolysis of pretreated sugarcane bagasse by organosolv process. The combination with easily hydrolyzable cellulose allows obtaining highly pure glucose and the reuse of enzymes. The immobilized enzyme represents most of the solid recovered at the end of the hydrolysis because almost all the solid substrate added to the reactor is solubilized.

Tipo: Folders

Palavras-chave: Sugarcane bagasse; Enzymatic hydrolysis; Glucose.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1132998

Biological Sciences

Oliveira, Luciana Ribeiro Coutinho; Universidade Estadual do Norte Fluminense; Barbosa, João Batista; Universidade Estadual do Norte Fluminense; Martins, Meire Lelis Leal; Universidade Estadual do Norte Fluminense; Martins, Marco Antonio; Universidade Estadual do Norte Fluminense.

Nowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its...

Filamentous fungi and media for cellulase production in solid state cultures

Palavras-chave: 5.07.03.00-9 Avicelase; Corn steep liquor; Sugarcane bagasse; Bacillus sp Tecnologia de Alimentos.

Ano: 2014

URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827

Kilikian,B.V.; Afonso,L.C.; Souza,T.F.C.; Ferreira,R.G.; Pinheiro,I.R..

Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly...

Lignocellulosic biomass: autohydrolysis pretreatment of lignocellulosic biomass.

Palavras-chave: Cellulase; Productivity; Solid media; Sugarcane bagasse; Myceliophtora sp.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100041

New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract

CARVALHO, F. B. de P..

A process of pretreatment and fractionation of lignocellulosic biomass by the use of water at high temperatures and pressures, to remove hemicellulose with little degradation of cellulose.

Tipo: Folders

Palavras-chave: Lignocellulosic; Sugarcane bagasse; Hydrolysis; Hemicellulose; Renewable resources.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133096

Silva,Debora Danielle Virginio da; Cândido,Elisangela de Jesus; Arruda,Priscila Vaz de; Silva,Silvio Silvério da; Felipe,Maria das Graças de Almeida.

The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia) stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments were carried out with hydrolysate supplemented with rice bran extract and (NH4)2SO4; peptone and yeast extract; (NH4)2SO4,...

Production of cellulases by Penicillium oxalicum through solid state fermentation using agroindustrial substrates

Palavras-chave: Candida guilliermondii; Hemicellulosic hydrolysate; Scheffersomyces stipitis; Rice bran extract; Sugarcane bagasse.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400043

Biological Sciences

Bomtempo, Fabrícia Vieira Silva; Santin, Franciane Maria Mantovani; Pimenta, Raphael Sanzio; Oliveira, Deyla Paula de; Guarda, Emerson Adriano.

The purpose of this study was to define the factors that influence the production of cellulases by Penicillium oxalicum, a strain obtained from a leaf-cutting ant colony and identified based on the ITS gene. The experimental design included solid state fermentation using sugarcane bagasse and lignocellulosic sorghum as the lignocellulosic substrate. The variables were analyzed using a 25-1 fractional factorial design, with three replicates on the central point. All the variables analyzed influenced the production of at least one of the three cellulose types analyzed. The highest values observed were: FPase 4.2 U g-1, CMCase 9.2 U g-1 and Avicelase 8.4 U g-1 using lignocellulosic sorghum as the substrate. The best conditions for enzyme production were:...

Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products

Palavras-chave: Sugarcane bagasse; Lignocellulosic sorghum; Cellulolytic enzymes. Microbiologia.

Ano: 2017

URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32384

BABT

Santos,Édilla Ribeiro dos; Teles,Zozilene Nascimento Santos; Campos,Núria Mariana; Souza,Diogo Angeli Jacinto de; Bispo,Aline Simões da Rocha; Nascimento,Rodrigo Pires do.

Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged...

Shiitake (lentinula edodes) production on a sterilized bagasse substrate enriched with rice bran and sugarcane molasses

Palavras-chave: Α-amylases; Sugarcane bagasse; Sisal waste; Streptomyces sp.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020

Rossi,Ivan Henrique; Monteiro,Antonio Carlos; Machado,José Octavio; Andrioli,João Luciano; Barbosa,José Carlos.

This investigation was performed to evaluate the biological efficiency (BE), mean mushroom weight (MMW), mean number of mushroom (MNM) and mushroom quality of Shiitake [ Lentinula edodes (Berk.) Pegler] when grown on a sterilized substrate composed by sugarcane bagasse enriched with rice bran and sugarcane molasses. The proportions of rice bran were 0, 15, 20, 25 and 30% (dry weight/dry weight of bagasse); and the concentrations of sugarcane molasses were 0, 30 and 60 g/kg (dry weight/dry weight of bagasse plus rice bran). Four flushes were obtained during the production cycle, providing 3 accumulated productions which were used for production analysis. The substrate supplemented with 25 and 30% rice bran yielded the highest BE (98.42 and 99.84%,...

Supplementation of sugarcane bagasse with rice bran and sugarcane molasses for shiitake (Lentinula edodes) spawn production

Palavras-chave: Lentinula edodes; Shiitake; Sugarcane bagasse.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100014

Rossi,Ivan Henrique; Monteiro,Antonio Carlos; Machado,José Octavio; Barbosa,José Carlos.

The purpose of this study was to assess the myceliation rate, mycelial vigor and "estimated biomass" of Lentinula edodes (Berk.) Pegler, grown on a sugarcane bagasse substrate enriched with rice bran and sugarcane molasses for spawn production. The proportions of rice bran used were 0, 10, 15, 20, 25, 30 and 40% (dry weight/dry weight of bagasse) and the sugarcane molasses concentrations tested were 0, 10, 20, 30, 40, 50 and 60 g/kg (dry weight/dry weight of bagasse plus rice bran). The myceliation rate was decreased by the addition of the higher quantities of rice bran. The 25 and 30% rice bran proportions induced the highest stimulation of mycelial vigor. The addition of sugarcane molasses did not change myceliation rate or mycelial vigor. The "estimated...

Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production

Palavras-chave: Lentinula edodes; Shiitake; Sugarcane bagasse.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100013

Santos,Bruna Silveira Lamanes dos; Gomes,Arthur Filipe Sousa; Franciscon,Emanuele Giuliane; Oliveira,Jean Maikon de; Baffi,Milla Alves.

Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus (SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at pH 3.5 and 55 °C (141...

USE OF RESIDUES FROM THE CELLULOSE INDUSTRY AND SUGARCANE BAGASSE IN PARTICLEBOARDS

Palavras-chave: Β-glucosidase; Β-xylosidase; Xylanase; Aspergillus; Sugarcane bagasse.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300903

REA

da Silva,Sérgio A. M.; Minillo,Larissa Q.; Aquino,Vinícius B. de M.; Lahr,Francisco A. R.; Christoforo,André L..

ABSTRACT The use of alternative materials such as lignocellulosic residues in the production of particleboards has increased considering that these residues are produced in large volumes and often do not have an appropriate destination. This research studied the use of residues from cellulose industries, sugarcane bagasse, and castor oil-based polyurethane resin in the production of wooden panels and evaluated the influence of using these residues on the physical and mechanical properties of the panels. The products were manufactured according to the Brazilian standard ABNT NBR 14810 and the requirements of the panels were evaluated based on national and international standards. All treatments partially met the regulatory requirements. The addition of...

Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse

Palavras-chave: Industrial residues; Eucalyptus; Sugarcane bagasse; Particleboards; Castor oil-based resin.

Ano: 2021

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162021000100107

Rezende,Maria Inês; Barbosa,Aneli de Melo; Vasconcelos,Ana Flora Dalberto; Endo,Asae Sakurada.

Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and...

Xylitol: xylitol production from sugarcane bagasse.

Palavras-chave: Trichoderma harzianum; Xylanase; Solid state fermentation; Sugarcane bagasse.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014